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Cy5.5
作者：德尔塔 日期：2022-09-27
Cy5.5 (Sulfo-Cyanine5.5) 是用于标记生物分子的近红外荧光染料 (Ex=673 nm, Em=707 nm)，如肽，蛋白质和寡核苷酸。Cy5.5 (Sulfo-Cyanine5.5) is a near-infrared fluorescent dye (Ex=673 nm, Em=707 nm) used to label biological molecules, such as peptides, proteins, and oligonucleotides[1].[1]. Zhu S, et al. Visualizing cancer and response to therapy in vivo using Cy5.5-labeled factor VIIa and anti-tissue factor antibody. J Drug Target. 2015 Apr;23(3):257-65. [2]. Jiang L, et al. pH/temperature sensitive magnetic nanogels conjugated with Cy5.5-labled lactoferrin for MR and fluorescence imaging of glioma in rats. Biomaterials. 2013 Oct;34(30):7418-28. [3]. Lim B, et al. A Unique Recombinant Fluoroprobe Targeting Activated Platelets Allows In Vivo Detection of Arterial Thrombosis and Pulmonary Embolism Using a Novel Three-Dimensional Fluorescence Emission Computed Tomography (FLECT) Technology. Theranostics. 2017 Feb 26;7(5):1047-1061.Cy5.5 (Sulfo-Cyanine5.5) is a near-infrared fluorescent dye (Ex=673 nm, Em=707 nm) used to label biological molecules, such as peptides, proteins, and oligonucleotides[1].[1]. Zhu S, et al. Visualizing cancer and response to therapy in vivo using Cy5.5-labeled factor VIIa and anti-tissue factor antibody. J Drug Target. 2015 Apr;23(3):257-65. [2]. Jiang L, et al. pH/temperature sensitive magnetic nanogels conjugated with Cy5.5-labled lactoferrin for MR and fluorescence imaging of glioma in rats. Biomaterials. 2013 Oct;34(30):7418-28. [3]. Lim B, et al. A Unique Recombinant Fluoroprobe Targeting Activated Platelets Allows In Vivo Detection of Arterial Thrombo
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CY5-YNE
作者：德尔塔 日期：2022-09-27
CY5-YNE是一种用于标记肽,蛋白质,寡核苷酸的氨基基团的反应染料。CY5-YNE is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.CY5-YNE is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.Cas No. 1345823-20-2 SDF 别名 N/A 化学名 N/A Canonical SMILES CC(/C(N1CCCCCC(NCC#C)=O)=C\C=C\C=C\C2=[N+](CC)C(C=CC(S(=O)([O-])=O)=C3)=C3C2(C)C)(C)C4=C1C=CC(S(=O)(O)=O)=C4 分子式 C36H43N3O7S2 分子量 693.87 溶解度 DMSO : ≥ 43 mg/mL (61.97 mM) 储存条件 Store at -20°C General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. Shipping Condition Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
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Cy5-SE (triethylamine salt)
作者：德尔塔 日期：2022-09-27
Cy5-SE is a hydrophilic amine-reactive fluorescent probe.1 It displays excitation/emission maxima of 646/662 nm, respectively. Cy5-SE-conjugated ligands have been used in the characterization of hematopoietic tumor microenvironments.1.Soodgupta, D., Zhou, H., Beaino, W., et al.Ex vivo and in vivo evaluation of overexpressed VLA-4 in multiple myeloma using LLP2A imaging agentsJ. Nucl. Med.57(4)640-645(2016)Cy5-SE is a hydrophilic amine-reactive fluorescent probe.1 It displays excitation/emission maxima of 646/662 nm, respectively. Cy5-SE-conjugated ligands have been used in the characterization of hematopoietic tumor microenvironments.1.Soodgupta, D., Zhou, H., Beaino, W., et al.Ex vivo and in vivo evaluation of overexpressed VLA-4 in multiple myeloma using LLP2A imaging agentsJ. Nucl. Med.57(4)640-645(2016)Cas No. 1497420-70-8 SDF 别名 N/A 化学名 N/A Canonical SMILES O=C(CCCCCN1/C(C(C)(C2=C1C=CC(S(O)(=O)=O)=C2)C)=C/C=C/C=C/C3=[N+](C4=CC=C(C=C4C3(C)C)S([O-])(=O)=O)CC)ON5C(CCC5=O)=O.CCN(CC)CC 分子式 C37H43N3O10S2.C6H15N 分子量 855.1 溶解度 DMF: 30 mg/ml,DMSO: 30 mg/ml,Ethanol: 15 mg/ml,PBS (pH 7.2): 1 mg/ml 储存条件 Store at -20°C General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it
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CY5-SE
作者：德尔塔 日期：2022-09-27
Cy5-SE是一种用于标记肽, 蛋白质, 寡核苷酸的氨基基团的反应染料。激发波长 (nm): 649, 发射波长 (nm): 670。Cy5-SE is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling.Excitation (nm):649, Emission (nm): 670.Cy5-SE is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling.Excitation (nm):649, Emission (nm): 670.Cas No. 146368-14-1 SDF 别名 Cy5 NHS Ester 化学名 N/A Canonical SMILES CC(/C(N1CCCCCC(ON2C(CCC2=O)=O)=O)=C\C=C\C=C\C3=[N+](CC)C(C=CC(S(=O)([O-])=O)=C4)=C4C3(C)C)(C)C5=C1C=CC(S(=O)(O)=O)=C5 分子式 C37H43N3O10S2 分子量 753.88 溶解度 DMSO: 25 mg/mL (33.16 mM) 储存条件
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Cy5-PEG3-azide
作者：德尔塔 日期：2022-09-27
Cy5-PEG3-azide是一种PROTAClinker，属于PEG类。可用于合成PROTAC分子。Cy5-PEG3-azide is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1].PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1]. [1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562Cy5-PEG3-azide is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1].PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1]. [1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562Cas No. N/A SDF 别名 N/A 化学名 N/A Canonical SMILES CC(C1=CC=CC=C1N/2C)(C)C2=C/C=C/C=C/C3=[N+](CCCCCC(NCCOCCOCCOCCN=[N+]=[N-])=O)C(C=CC=C4)=C4C3(C)C.[Cl-] 分子式 C40H55ClN6O4 分子量 719.36
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CY5-N3
作者：德尔塔 日期：2022-09-27
CY5-N3是一种Cy5-叠氮化物，是一种荧光染料。CY5-N3 is a Cy5-azide, which is a fluorescent dye.CY5-N3 can be used to monitor the CuAAC of branched DNA with Cy5-azide by HPLC[1].[1]. Finke A, et al. Designer Extracellular Matrix Based on DNA-Peptide Networks Generated by Polymerase ChainReaction. Angew Chem Int Ed Engl. 2016 Aug 16;55(34):10136-40.CY5-N3 is a Cy5-azide, which is a fluorescent dye.CY5-N3 can be used to monitor the CuAAC of branched DNA with Cy5-azide by HPLC[1].[1]. Finke A, et al. Designer Extracellular Matrix Based on DNA-Peptide Networks Generated by Polymerase ChainReaction. Angew Chem Int Ed Engl. 2016 Aug 16;55(34):10136-40.Cas No. 1621101-43-6 SDF 别名 N/A 化学名 N/A Canonical SMILES CC1(C)/C(N(C2=CC=C(S(=O)(O)=O)C=C21)CCCCCC(NCCCN=[N+]=[N-])=O)=C\C=C\C=C\C(C(C)(C3=CC(S(=O)([O-])=O)=CC=C43)C)=[N+]4CC 分子式 C36H46N6O7S2 分子量 738.92 溶解度 DMSO : 25 mg/mL (33.83 mM) 储存条件 Store at -20°C,unstable in solution, ready to use. General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. Shipping Condition Evaluation sample solution : ship with blue ice
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Cy5 NHS ester(Et)
作者：德尔塔 日期：2022-09-27
氨基反应性荧光染料，溶于水Cas No. N/A SDF 别名 N/A 化学名 N/A Canonical SMILES CC1(C)C2=C(C=CC(S(=O)([O-])=[O-])=C2)[N+](CCCCCC(ON3C(CCC3=O)=O)=O)=C1/C=C/C=C/C=C4N(CC)C5=CC=C(S(=O)([O-])=O)C=C5C/4(C)C.[Na+] 分子式 C37H42N3NaO10S2 分子量 775.86 溶解度 insoluble in EtOH; >1.5 mg/mL in H2O with ultrasonic; >16.67 mg/mL in DMSO 储存条件 Store at -20°C General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. Shipping Condition Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
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Cy5 NHS ester (non-sulfonated)
作者：德尔塔 日期：2022-09-27
Labeling of amino-groups in biomolecules.Cyanine5 NHS ester is a ideal reactive dye for the labeling of peptides, proteins, and oligonucleotides which contain the amino-groups. Recently, among the life science research and diagnostics, Dye color quantity can be detected in gel electrophoresis by naked eye as the dye color is very intense and as small as 1 nanomol. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. For more sophisticated targets such as easily degradable proteins, when use of DMF or DMSO is undesirable, consider using water-soluble Cy5 NHS ester which does not require co-solvent, and has very similar fluorescent properties.In mice, Cy5-labeled polyplexes was intravenous injected to evaluate the targeting ability of polyplexes in tumor-bearing nude mice. Heart, liver, spleen, lung, kidney and tumor, were removed after the mice were sacrificed and the fluorescence intensities of Cy5-labeled polyplexes were immediately examined [1].Reference:[1] Chen, K. ; Chen, Q.; Wang, K.; Zhu, J.; Li, W.; Li, W.; Qiu, L.; Guan, G.; Qiao, M.; Zhao, X.; Hu, H.; Chen, D. Synthesis and characterization of a PAMAM-OH derivative containing an acid-labile β-thiopropionate bond for gene delivery. International Journal of Pharmaceutics, 2016, 509(1–2), 314–327.Cyanine5 NHS ester is a ideal reactive dye for the labeling of peptides, protei
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Cy5 NHS ester
作者：德尔塔 日期：2022-09-27
Cy5 NHS ester(Cyanine5 NHS ester) is a Red emitting fluorescent dye for labeling of amino-groups in peptides, proteins, and oligonucleotides.Labeling experiment [1]:Preparation Method1 mg of Cy5 NHS ester dye was dissolved in 0.1 ml of 62.5 mM sodium tetraborate buffer (pH 8.5). After adding L-methionine in saturation, the mixture was incubated overnight at 4 °C. Fluorophore (Cy5)-conjugated methionine (Cy5-Met) was purified by reverse-phase chromatography on an HPLC system using a C18 column.Reaction Conditions1 mg Cy5 NHS in 0.1 ml sodium tetraborate bufferApplicationsFluorophore-conjugated methionine was prepared by conjugation of the alpha amino group of L-methionine with Cyanine5 (Cy5) NHS ester dye.References:[1]. Kim JM, Seong BL. Highly chromophoric Cy5-methionine for N-terminal fluorescent tagging of proteins in eukaryotic translation systems. Sci Rep. 2017 Sep 14;7(1):11642.Labeling experiment [1]:Preparation Method1 mg of Cy5 NHS ester dye was dissolved in 0.1 ml of 62.5 mM sodium tetraborate buffer (pH 8.5). After adding L-methionine in saturation, the mixture was incubated overnight at 4 °C. Fluorophore (Cy5)-conjugated methionine (Cy5-Met) was purified by reverse-phase chromatography on an HPLC system using a C18 column.Reaction Conditions1 mg Cy5 NHS in 0.1 ml sodium tetraborate bufferApplicationsFluorophore-conjugated methionine was prepared by conjugation of the alpha amino group of L-methionine with Cyanine5 (Cy5) NHS ester dye.References:[1]. Kim JM, Seong BL. Highly chromophoric Cy5-methionine for N-terminal fluorescent tagging of proteins in eukaryotic
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Cy5 maleimide (non-sulfonated)
作者：德尔塔 日期：2022-09-27
Labeling of cysteine residues, as well as other thiolated moleculesCy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups. The labeling reagent is a common fluorophore dye with low aqueous solubility which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers. Cy5 maleimide is part of the probe among the biological research. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. The user can generate probes with virtually any non-genetically encoded moiety of interest with the scaffold in hand.G3C(Cy5) probe consists of two parts. First, a GGGC scaffold peptide is synthesized on solid phase, followed by coupling of the liberated peptide to Cy5 maleimide, while in solution [1].Reference:[1]. Popp, M.W. Site-Specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist. Methods in Molecular Biology,2015, 1266, 185–198.Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups. The labeling reagent is a common fluorophore dye with low aqueous solubility which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers. Cy5 maleimide is part of the probe among the biological research. For biomolecule labeling, the labeling reag
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Cy5 hydrazide (non-sulfonated)
作者：德尔塔 日期：2022-09-27
carbonyl-reactive dyeCy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones. The dye examples are proteins subjected to oxidative stress, glycoproteins pre-activated by periodate oxidation, and oligonucleotides with aldehyde moieties. Cy5 hydrazide could replace carbonyl-reactive Alexa Fluor 647 and DyLight 649 dyes. For biomolecule labeling, the labeling regent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.In HL-60 cells, Protein carbonylation induced by Hydrogen peroxide (H2O2) was quantitatively analyzed by using fluorescent dyes of Cy5-hydrazide dye, followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and fluorescence determination [1].Reference:[1] Yoshimura, T. ; Harashima, M.; Kurogi, K.; Suiko, M.; Liu, M.-C.; Sakakibara, Y. A novel procedure for the assessment of the antioxidant capacity of food components. Analytical Biochemistry, 2016, 507, 7–12.Cy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones. The dye examples are proteins subjected to oxidative stress, glycoproteins pre-activated by periodate oxidation, and oligonucleotides
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Cy5 carboxylic acid(Et)
作者：德尔塔 日期：2022-09-27
非反应性荧光团，用于对照实验和校准 ,溶于水Cas No. N/A SDF 别名 N/A 化学名 N/A Canonical SMILES CC1(C)C2=C(C=CC(S(=O)([O-])=O)=C2)[N+](CCCCCC(O)=O)=C1/C=C/C=C/C=C3N(CC)C4=CC=C(S(=O)([O-])=O)C=C4C/3(C)C.[Na+] 分子式 C33H39N2NaO8S2 分子量 678.79 溶解度 >67.9 mg/mL in H2O; >6.83 mg/mL in EtOH; >8.56 mg/mL in DMSO 储存条件 Store at -20°C General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. Shipping Condition Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
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Cy5 carboxylic acid (non-sulfonated)
作者：德尔塔 日期：2022-09-27
Non-reactive fluorophore, for control experiments, and for calibrationCy5 carboxylic acid is a non-activated carboxylic acid with low aqueous solubility. For coupling with amines, or protein labeling, Cy5 NHS ester and water-soluble sulfo-Cy5 NHS ester is preferred. This labeling dye has limited water solubility. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. In the cells, The Cy5 carboxylic acid label on the dye can track the efficiency of the protein expression [1].Reference:[1] Deyle, K. M.; Farrow, B.; Hee, Y.Q.; Work, J.; Wong, M.; Lai, B.; Umeda, A.; Millward, S.W.; Nag, A.; Das, S.; Heath, J.R. A protein-targeting strategy used to develop a selective inhibitor of the E17K point mutation in the PH domain of Akt1. Nature Chemistry,2015, 7, 455–462.Cy5 carboxylic acid is a non-activated carboxylic acid with low aqueous solubility. For coupling with amines, or protein labeling, Cy5 NHS ester and water-soluble sulfo-Cy5 NHS ester is preferred. This labeling dye has limited water solubility. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. In the cells, The Cy5 carboxylic acid
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Cy5 carboxylic acid
作者：德尔塔 日期：2022-09-27
Non-reactive fluorophore, for control experiments, and for calibrationSulfo-Cy5 carboxylic acid is a non-activated, sulfonated, hydrophilic and highly water soluble dye. Non-Sulfo-Cy5 carboxylic acid analog is available. For kinds of researches related experiments, using pre-activated sulfo-Cy5 NHS ester is preferred. To labeling proteins and peptides, no organic co-solvent is needed. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions. In the soma of SNc DA neurons, to examine whether cyamemazine accumulates in the soma of SNc DA neurons, ovoid neurons were patch clamped and filled with the Sulfo-Cy5 carboxylic acid. [1].Reference:[1] Tucker, K. R.; Block, E.R.; Levitan, E.S. Action potentials and amphetamine release antipsychotic drug from dopamine neuron synaptic VMAT vesicles. Proceedings of the National Academy of Sciences of the U.S.A., 2015, 112(32), E4485–E4494.Sulfo-Cy5 carboxylic acid is a non-activated, sulfonated, hydrophilic and highly water soluble dye. Non-Sulfo-Cy5 carboxylic acid analog is available. For kinds of researches related experiments, using pre-activated sulfo-Cy5 NHS ester is preferred. To labeling proteins and peptides, no organic co-solvent is needed. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions. In the soma of SNc DA neurons, to examine whether cyamemazine accumulates in the soma of SNc DA neurons, ovoid neurons were patch clamped and fil
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Cy5 Boc-hydrazide (non-sulfonated)
作者：德尔塔 日期：2022-09-27
carbonyl-reactive dyeBoc-protected Cy5 hydrazide is a reactive dye for the labeling of aldehydes and ketones with high aqueous solubility. Trifluoroacetic acid or other strong acid can remove the Boc-(tert-butoxycarbonyl) protective group. Reactive hydrazide can couple with various carbonyl groups yielding Cy5-labeled molecules. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.Boc-protected Cy5 hydrazide is a reactive dye for the labeling of aldehydes and ketones with high aqueous solubility. Trifluoroacetic acid or other strong acid can remove the Boc-(tert-butoxycarbonyl) protective group. Reactive hydrazide can couple with various carbonyl groups yielding Cy5-labeled molecules. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.Cas No. N/A SDF 别名 N/A 化学名 1-(6-(2-(tert-butoxycarbonyl)hydrazinyl)-6-oxohexyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3H-indol-1-ium Canonic
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