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Cy3 maleimide (non-sulfonated)
作者：德尔塔 日期：2022-09-26
Labeling of cysteine residues, as well as other thiolated moleculesCy3 maleimide is a selective and efficient fluorophore dye with low aqueous solubility. This labeling reagent can be used to attach Cy3 fluorophore to proteins and peptides which contain cysteine residues, as well as to other thiolate molecules (such as thiol-containing oligonucleotides). Before the labeling reactions, Cystines should be reduced with TCEP (tris-carboxyethylphosphine). For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. MSNs were labeled by Cy3 maleimide named MSN-Cy3s at the concentration of 10 μg ml-1. MSN-Cy3s incubated with MEF-mEGFP cells on a glass coverslip in culture medium for 2 h To further visualize the MSN locations in the cytosol we used super-resolution microscopy (3D SIM). The visually microscope image results confirm that MSNs were internalized by MEF-mEGFP cells [1].Reference:[1]. Chiu, H.-Y.; Deng, W.; Engelke, H.; Helma, J.; Leonhardt, H.; Bein, T. Intracellular chromobody delivery by mesoporous silica nanoparticles for antigen targeting and visualization in real time. Scientific Reports, 2016, 6, 25019.Cy3 maleimide is a selective and efficient fluorophore dye with low aqueous solubility. This labeling reagent can be used to attach Cy3 fluorophore to proteins and peptides which contain cysteine residues, as well as to other th
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Cy3 hydrazide (non-sulfonated)
作者：德尔塔 日期：2022-09-26
carbonyl-reactive dyeCy3 hydrazide is a carbonyl-reactive dye, an analog of Cy3® hydrazide.Cyanine3 dyes have been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc.Cy3 hydrazide can be used to label various carbonyl-containing molecules such as antibodies and other glycoproteins after periodate oxidation, proteins which have undergone deamination or oxidative stress, or reducing saccarides. Cyanine3 is compatible with a number of fluorescent instruments.References:[1]. Pan D, Qin H, Cooperman BS. Synthesis and functional activity of tRNAs labeled with fluorescent hydrazides in the D-loop. RNA. 2009 Feb;15(2):346-54.[2]. Tamarit J, de Hoogh A, Obis E, et al. Analysis of oxidative stress-induced protein carbonylation using fluorescent hydrazides. J Proteomics. 2012 Jun 27;75(12):3778-88.[3]. Kameyama A, Kaneda Y, Yamanaka H, et al. Detection of oligosaccharides labeled with cyanine dyes using matrix-assisted laser desorption/ionization mass spectrometry. Anal Chem. 2004 Aug 1;76(15):4537-42.Cy3 hydrazide is a carbonyl-reactive dye, an analog of Cy3® hydrazide.Cyanine3 dyes have been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc.Cy3 hydrazide can be used to label various carbonyl-containing molecules such as antibodies and other glycoproteins after periodate oxidation, proteins which have undergone deamination or ox
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Cy3 carboxylic acid(Et)
作者：德尔塔 日期：2022-09-26
非反应性荧光团，用于对照实验和校准 ,溶于水Cas No. N/A SDF 别名 N/A 化学名 N/A Canonical SMILES CC(C1=CC(S(=O)([O-])=[O-])=CC=C1N/2CC)(C)C2=C/C=C/C3=[N+](CCCCCC(O)=O)C(C=CC(S(=O)([O-])=[O-])=C4)=C4C3(C)C.[Na+] 分子式 C31H37N2NaO8S2 分子量 652.75 溶解度 >65.3 mg/mL in H2O; >17.63 mg/mL in EtOH with ultrasonic; >9.54 mg/mL in DMSO 储存条件 Store at -20°C General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. Shipping Condition Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
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Cy3 carboxylic acid (non-sulfonated)
作者：德尔塔 日期：2022-09-26
Non-reactive fluorophore, for control experiments, and for calibrationCy3 carboxylic acid is a non-activated fluorescence dye. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. This Non-sulfonated reagent has limited aqueous solubility, but with good solubility in organic solvents. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. In the supramolecular glutamine assay, Cy3 carboxylic acid was conjugated to a single strain DNA and then immobilized onto the glass slide through DNA hybridization. The Cy3 groups serve as FRET donor and the cyclodextrins as supramolecular host [1]. Reference:[1] Xue, M. ; Wei, W.; Su, Y.; Johnson, D.; Heath, J.R. Supramolecular Probes for Assessing Glutamine Uptake Enable Semi-Quantitative Metabolic Models in Single Cells. Journal of the American Chemical Society, 2016, 138(9), 3085–3093.Cy3 carboxylic acid is a non-activated fluorescence dye. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. This Non-sulfonated reagent has limited aqueous solubility, but with good solubility in organic solvents. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient r
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Cy3 Bis NHS ester
作者：德尔塔 日期：2022-09-26
荧光染料，含有两个氨基反应基团,溶于水Cas No. N/A SDF 别名 N/A 化学名 N/A Canonical SMILES CC(C1=CC(S(=O)([O-])=[O-])=CC=C1N/2CCCCCC(ON3C(CCC3=O)=O)=O)(C)C2=C/C=C/C4=[N+](CCCCCC(ON5C(CCC5=O)=O)=O)C(C=CC(S(=O)([O-])=[O-])=C6)=C6C4(C)C.[Na+] 分子式 C43H49N4NaO14S2 分子量 932.99 溶解度 insoluble in EtOH; >41 mg/mL in DMSO; >54.6 mg/mL in H2O 储存条件 Store at -20°C General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. Shipping Condition Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
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Cy3 Bis carboxylic acid
作者：德尔塔 日期：2022-09-26
非反应性荧光团，用于对照实验和校准,溶于水Cas No. N/A SDF 别名 N/A 化学名 N/A Canonical SMILES CC(C1=CC(S(=O)([O-])=[O-])=CC=C1N/2CCCCCC(O)=O)(C)C2=C/C=C/C3=[N+](CCCCCC(O)=O)C(C=CC(S(=O)([O-])=[O-])=C4)=C4C3(C)C.[Na+] 分子式 C35H43N2NaO10S2 分子量 738.84 溶解度 Soluble in DMSO 储存条件 Store at -20°C General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. Shipping Condition Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
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Cy3 azide (non-sulfonated)
作者：德尔塔 日期：2022-09-26
used for Click chemistry to label alkyne-modified oligonucleotidesCy3 azide is a non-sulfonated dye ready for the use in Click Chemistry reaction which can be detected by various fluorometers, imagers, and microscopes. The reagent can be detected by naked eye on gels because of inherently high extinction coefficient. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. Product is available both as solid compound, and as 10 mM solution in DMSO. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. Water-soluble version of this reagent is also available. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. In protein analysis, proteins labeled by Cy3 azide couple with Cy5 azide can substantially simplifies the target identification process than only one fluorescent dye [1]. Reference:[1]. Park, H.; Koo, J.Y.; Srikanth, Y.V.V.; Oh, S.; Lee, J.; Park, J.; Park, S.B. Nonspecific protein labeling of photoaffinity linkers correlates with their molecular shapes in living cells. Chemical Communications, 2016, 52(34), 5828–5831.Cy3 azide is a non-sulfonated dye ready for the use in Click Chemistry reaction which can be detected by various fluorometers, imagers, and microscopes. The reagent can be detected by naked eye on gels because of inherently high extinction coefficient. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for
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Cy3 azide
作者：德尔塔 日期：2022-09-26
used for Click chemistry to label alkyne-modified oligonucleotidesSulfo-Cy3 azide is a sulfonated, hydrophilic and highly water soluble dye for Click Chemistry. For labeling of various molecules in aqueous phase for brightness and photostability of fluorophore, sulfo-Cy3 azide is an ideal choice. Click reaction can be carried out in pure water without need to use organic co-solvent. For labeling proteins and even intact biological objects, no organic co-solvent is needed. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions. In vitro, sulfo-Cy3 azide was used for fluorescent microscopy staining. Human U87MG glioblastoma cells that overexpress uPAR were just stained by Cy3-AE105 conjugate [1].Reference:[1] Sun, L. Gai, Y. Anderson, C.J.; Zeng, D. Highly-efficient and versatile fluorous-tagged Cu (I)-catalyzed azide–alkyne cycloaddition ligand for preparing bioconjugates. Chemical Communications, 2015, 51, 17072–17075.Sulfo-Cy3 azide is a sulfonated, hydrophilic and highly water soluble dye for Click Chemistry. For labeling of various molecules in aqueous phase for brightness and photostability of fluorophore, sulfo-Cy3 azide is an ideal choice. Click reaction can be carried out in pure water without need to use organic co-solvent. For labeling proteins and even intact biological objects, no organic co-solvent is needed. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions. In v
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Cy3 alkyne (non-sulfonated)
作者：德尔塔 日期：2022-09-26
used for Click chemistry to label any molecule bearing azide group.Cy3 alkyne is a water-insoluble dye ready for the use in Click Chemistry reaction. With this bright and photostable reagent, molecules bearing azide group can be attached by it. The reagent can be efficiently attached to water-soluble molecules such as proteins when used with organic co-solvent (DMSO or DMF). For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. In dendritic cells, the research confirming that the conjugates of Cy3-alkyne and Cy5-BSA-pDMDOMA particles are internalized by the cells rather than sticking to the cell surface. Additionally, confocal microscopy imaging shows a strong co-localization of the Cy3 and Cy5 channel [1]. Reference:[1] Vanparijs, N. ; De Coen, R.; Laplace, D.R.; Louage, B.; Maji, S.; Lybaert, L.; Hoogenboom, R.; De Geest, B. Transiently responsive protein-polymer conjugates via a grafting-from RAFT approach: for intracellular co-delivery of proteins and immune-modulators.Chemical Communications, 2015, 51, 13972–13975.Cy3 alkyne is a water-insoluble dye ready for the use in Click Chemistry reaction. With this bright and photostable reagent, molecules bearing azide group can be attached by it. The reagent can be efficiently attached to water-soluble molecule
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Cyanine5.5 tetrazine
作者：德尔塔 日期：2022-09-26
Cyanine5.5 is a far red emitting dye for in vivo imaging and applications requiring low background. This Cyanine5.5 derivative contains tetrazine moiety that reacts with trans-cyclooctenes, cyclopropenes, and some strained cyclooctynes with the formation of stable conjugates.This labeling reaction is extremely fast, and can be used in vivo.Cyanine5.5 is a far red emitting dye for in vivo imaging and applications requiring low background. This Cyanine5.5 derivative contains tetrazine moiety that reacts with trans-cyclooctenes, cyclopropenes, and some strained cyclooctynes with the formation of stable conjugates.This labeling reaction is extremely fast, and can be used in vivo.Cas No. N/A SDF 别名 N/A 化学名 N/A Canonical SMILES N/A 分子式 C50H52N7F6OP 分子量 911.96 溶解度 good in DCM, DMF, DMSO 储存条件 Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. Shipping Condition Evaluation sample solution : ship with blue ice
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Cyanine5.5 NHS ester
作者：德尔塔 日期：2022-09-26
Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester.Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.This reagent can replace NHS esters of Cy5.5®, Alexa Fluor 680, and DyLight 680.Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester.Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.This reagent can replace NHS esters of Cy5.5®, Alexa Fluor 680, and DyLight 680.Cas No. 2375105-86-3 SDF 别名 N/A 化学名 N/A Canonical SMILES N/A 分子式 C44H46N3BF4O4 分子量 767.66 溶解度 soluble in organic solvents (DMSO, DMF, dichloromethane), practically insoluble in water (< 1 uM, < 1 mg/L) 储存条件 Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. General tips
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Cyanine5.5 maleimide
作者：德尔塔 日期：2022-09-26
Cyanine5.5 maleimide is a thiol reactive dye which is capable of selective labeling of sulfhydryl groups in proteins, an analog of Cy5.5® maleimide.Near infrared emission of Cyanine5.5 makes this dye suitable for bioimaging applications.Cyanine5.5 can replace Cy5.5®, Alexa Fluor 680, and DyLight 680.Cyanine5.5 maleimide is a thiol reactive dye which is capable of selective labeling of sulfhydryl groups in proteins, an analog of Cy5.5® maleimide.Near infrared emission of Cyanine5.5 makes this dye suitable for bioimaging applications.Cyanine5.5 can replace Cy5.5®, Alexa Fluor 680, and DyLight 680.Cas No. 1593644-50-8 SDF 别名 N/A 化学名 N/A Canonical SMILES N/A 分子式 C46H49ClN4O3 分子量 741.36 溶解度 soluble in organic solvents (DMSO, DMF, dichloromethane), practically insoluble in water (< 1 uM, < 1 mg/L) 储存条件 Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. Shipping Condition Evaluation sample solution : ship with blue ice
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Cyanine5.5 hydrazide
作者：德尔塔 日期：2022-09-26
Cyanine5.5 hydrazide is a carbonyl-reactive near infrared dye. Hydrazides quickly react with carbonyl compounds, such as aldehydes and ketones, and form stable hydrazones. This reaction is useful for the preparation of labeled conjugates.Carbonyl groups in biomolecules can form spontaneously after oxidative stress or deamination of proteins. Alternatively, aldehyde groups can be specifically generated by periodate oxidation of sugar residues of glycoproteins, for example antibodies. Antibodies can be oxidized by sodium periodate and labeled with Cyanine5.5 hydrazide to form Cyanine5.5 labeled antibodies. Since sugar residues in antibodies are located far from variable, epitope-binding site, this method is particularly good for the labeling of antibodies.Cyanine5.5 is a near infrared fluorophore, an analog of Cy5.5®. It is particularly suitable for the applications which require low fluorescence background. The absorption and emission wavelength of the dye exhibit significant tissue penetration, so Cyanine5.5 is suitable for in vivo NIR imaging applications.Cyanine5.5 hydrazide is a carbonyl-reactive near infrared dye. Hydrazides quickly react with carbonyl compounds, such as aldehydes and ketones, and form stable hydrazones. This reaction is useful for the preparation of labeled conjugates.Carbonyl groups in biomolecules can form spontaneously after oxidative stress or deamination of proteins. Alternatively, aldehyde groups can be specifically generated by periodate oxidation of sugar residues of glycoproteins, for example antibodies. Antibodies can be oxidized by sodium pe
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Cyanine5.5 DBCO
作者：德尔塔 日期：2022-09-26
Strain promoted alkyne azide cycloaddition (spAAc) between strained cycloalkynes and azides is a useful bioconjugation method that eliminates the need for copper catalyst necessary for classical CuAAc Click chemistry.Cyanine5.5 DBCO is a cyclooctyne product containing Cyanine5.5 fluorescent dye. This fluorophore is used in bioimaging, biodistribution studies, and microscopy.Cyanine5.5 DBCO can be used to conjugate the fluorophore with various azides.Strain promoted alkyne azide cycloaddition (spAAc) between strained cycloalkynes and azides is a useful bioconjugation method that eliminates the need for copper catalyst necessary for classical CuAAc Click chemistry.Cyanine5.5 DBCO is a cyclooctyne product containing Cyanine5.5 fluorescent dye. This fluorophore is used in bioimaging, biodistribution studies, and microscopy.Cyanine5.5 DBCO can be used to conjugate the fluorophore with various azides.Cas No. N/A SDF 别名 N/A 化学名 N/A Canonical SMILES N/A 分子式 C61H63N4F6O2P 分子量 1029.14 溶解度 good in DMF, DMSO, chlorinated organic solvents 储存条件 Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. General tips Fo
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Cyanine5.5-N3,CY5.5 azide
作者：德尔塔 日期：2022-09-23
Cyanine5.5-N3,CY5.5 azideCyanine5.5 azide标记试剂用于点击化学，有10mM溶解于DMSO和粉剂两种形式选择。该叠氮类染料溶于有机溶剂，所以标记反应需要有少量有机共溶剂的存在。该染料可用于在水和有机溶剂的混合物中标记炔烃修饰的生物分子。溶解在DMSO中的溶液形成是用于这种生物偶联的即用溶液。水溶液的该染料的产品：Sulfo-Cyanine5 azide我公司生产各种荧光染料及活性荧光染料，产品质量稳定，纯度大于97%，品质保证，售后无忧，价格实惠，国内性价比的荧光染料。The NIR-fluorescent Cy5.5 azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. Cy5.5 is a water-soluble, pH-insensitive, bright fluorescent probe that can be used in place of Alexa Fluor® 680, DyLigh®t 680, or IRDye™ 680 and other dyes excitable at a wavelength at around 680 nm.Cyanine5.5-N3,CY5.5 azide Abs/Em Maxima 678/694 nmExtinction coefficient 190,000Spectrally similar dyes Alexa Fluor® 680, IRDye® 680RD, CF™ 680 Dye, DyLight™ 680Molecular weight 999.15CAS N/ASolubility Water, DMSO, DMFPurity >95% (HPLC)Appearance Blue solidStorage conditions -20CShipping conditions Ambient temperature 其他荧光产品：Cyanine3 alkyneCy3 alkyneCyanine3 azideCy3 azide Cy3 N3Cyanine3 carboxylic acid 1144107-76-5Cy3 carboxylic acidCyanine3 hydrazideCyanine3 maleimideCy3 maleimideCyanine3 NHS ester 1032678-38-8Cy3 NHS esterCyanine3.5 azideCyanine3.5 carboxylic acid 1144107-79-8Cyanine3.5 NHS esterCy3.5 NHS esterCyanine5 alkyneCyanine5
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