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PF-670462 分子式：C19H22Cl2FN5分子量：410.32 简介： PF-670462是有效的、选择性CK1ε和CK1δ抑制剂，IC50分别为90 nM和13 nM。  物理性状及指标：  外观：……………………粉末  溶解性：…………………H2O ：28 mg/mL (68.24 mM)；Ethanol：82 mg/mL (199.84 mM) ；DMSO: 82 mg/mL (199.84 mM) 含量：……………………>98%  储存温度：-20℃，避光防潮密闭干燥 生物活性 描述 PF-670462是有效的、选择性CK1ε和CK1δ抑制剂，IC50分别为90 nM和13 nM。 IC50 & Target CK1δ  (Cell-free assay) CK1ε (Cell-free assay) 13 nM 90 nM 体外 PF-670462 is a 14 nM inhibitor of CK1δ in vitro and also potently inhibits p38 and EGFR. In vitro, PF670462 prevents TGF-β-induced epithelial-mesenchymal transition. 体内 PF670462 administered systemically or locally by inhalation prevents both acute and chronic bleomycin-induced pulmonary fibrosis in mice. 细胞实验 Cell lines: A549 cells Concentrations: 0.3-10 μM Incubation Time: 48 h Method: A549 cells are left to adhere overnight. Cells are then serum-starved for 16 h prior to pre-incubation with PF670462 (0.3-10 μM) for 30 min then TGF-β (100 pM) for 48 h. Cells are fixed in 10% neutral buffered formalin for 15 min and non-specific binding sites are blocked by incubation with 5% normal goat serum/0.3% Triton X-100 in PBS for 1 h. E-Cadherin expression is detected by immunofluorescence staining. (Only for Reference) 动物实验 Animal Models: Six- to eight-week old 20–25 g C57Bl/6 mice Formulation: initially dissolved in 100% DMSO and prepared for injection by 1:10 dilution in Arachis oil Dosages: 30 mg/kg/day Administration: IP (Only for Reference) 储液配置及储存： 按表中溶解性配置；如溶解困难，可以通过快速搅拌，超声或温和加热（在45-60°C下水浴）。液体稳定性报道的很少，建议现配现用，如需储存，建议：-20℃1-3月；-80℃ 3-6月。 浓度/体积/质量 1 mg 5 mg 10 mg 1 mM 2.4371 mL 12.1856 mL 24.3712 mL 5 mM 0.4874 mL 2.4371 mL 4.8742 mL 10 mM 0.2437 mL 1.2186 mL 2.4371 mL 50 mM 0.0487 mL 0.2437 mL 0.4874 mL 【注意 】 ●我司产品为非无菌包装，若用于细胞培养，请提前做预处理，除去热原细菌，否则会导致染菌。 ●部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，以上数据仅供参考交流研究之用。 我司所售出产品仅供于科研研究用途（非临床科研研究），每次销售产品行为都适用于我司网上所列明的通用销售条款。 CAS 号：950912-80-8 英文名字：PF670462 质量标准：>98%,BR 分子式：C19H20FN5.2(HCl) · HPLC pdf下载 >> · HNMR pdf下载 >>