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潮霉素B；Hygromycin B 分子式：C20H37N3O13    分子量：527.52 物理性状及指标： 外观：……………………类白色或微黄褐色粉末 溶解性：…………………易溶于水、甲醇和乙醇。 使用方法推荐（仅供参考） 储运液配置：溶解于无菌细胞培养级1M HEPES buffer (MA0036)或者PBS1X(MA0015)，配置成浓度为50mg/ml. 无菌微膜过滤。然后根据自身实验要求进行稀释或者使用 筛选条件：Most cells growing aerobically are killed by Hygromycin B in the concentration range of 50 to 500 μg/ml. However, the sensitivity of cells is pH dependent (i.e. the higher the pH of the culture medium the greater the sensitivity). Thus, the concentration of Hygromycin B required for complete growth inhibition of given cells can be reduced by increasing the pH of the medium. In addition, you can also lower the required amount of Hygromycin B Gold by using low‑salt media whenever possible. Escherichia coli (大肠杆菌) Hygromycin-resistant transformants are selected in low‑salt LB agar medium (yeast extract 5g/l, tryptone 10 g/l, NaCl 5 g/l, agar 15 g/l, pH 8) supplemented with 50 to 100 μg/ml of Hygromycin B. Plates containing Hygromycin B are stable for 1 month when stored at 4 °C. Mammalian cells （哺乳动物细胞） The working concentrations of Hygromycin B for mammalian cell lines vary from 50 to 200 μg/ml; in a few cases, up to 500 μg/ml. In a starting experiment we recommend to determine the optimal concentration of Hygromycin B required to kill your host cell line. Killing and detachment of dead cells from the plate, especially at high cell density, can require a longer time than with G418. Hygromycin‑resistant stable transfectants are usually obtained after 10 days to 3 weeks incubation, depending on the cell line. See table on the next page for suggested working concentrations of Hygromycin B in mammalian cells. 工作浓度 Hygromycin B normally used at a concentration of 200 μg/ml, a 500‑fold dilution from the stock solution. However, the optimal concentration needs to be determined for your cells. Suggested concentrations of Hygromycin B for selection in some examples of mammalian cells are listed below. Cell line Medium Hygromycin B conc. B16 (Mouse melanocytes) RPMI 100-200 μg/ml CHO (Chinese hamster ovarian cells) MEM 100-500 μg/ml HeLa (Human uterine cells) DMEM 100-200 μg/ml HEK293 (Human embryonic kidney cells) DMEM 50-400 μg/ml Raji (Human lymphocytes) RPMI 125-300 μg/ml THP-1 (Human monocytes) RPMI 250-400 μg/ml 用途及描述：科研试剂，阻止多肽合成和抑制延长。用于原核和真核细胞的选择和保藏。潮霉素B（Hygromycin B）是由吸水链霉菌（Streptomyces hygroscopicus）代谢产生的一种氨基糖苷类抗生素，通过干扰70S核糖体易位和诱导对mRNA模板的错读而抑制蛋白合成，从而杀死原核（如细菌）、真核（如酵母菌，真菌）和高等哺乳动物真核细胞。 储存条件：2-8℃，避光防潮密闭干燥。 运输条件：常温运输 相关产品推荐，美仑经典筛选抗生素系列 MB2005 嘌呤霉素二盐酸盐 Puromycin dihydrochloride MB6158 潮霉素B Hygromycin B MB2506 Blasticidin(灭瘟素S盐酸盐) Blasticidine S hydrochloride MB3575 Zeocin 粉末 Zeocin powder MB1733 G418硫酸盐,遗传霉素 G-418 disulfate,Geneticin 我司所售出产品仅供于科研研究用途（非临床科研研究），每次销售产品行为都适用于我司网上所列明的通用销售条款。 CAS 号：31282-04-9 英文名字：Hygromycin B 质量标准：≥90%,粉末,进分 TESTS SPECIFICATIONS Appearance Off-white powder Solubility 50mg/ml in water Identification HNMR Specific rotation +18.0 to +23.0° Activity ≥950U/mg Purity ≥90% 请输入货号: 请输入批号: · HNMR(J0... pdf下载 >> · HNMR pdf下载 >> 摩尔浓度计算器 （本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系） 摩尔浓度计算公式：质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol) 质量（g） = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol) /* function check(e) { var re = /^\d+(?=\.{0,1}\d+$|$)/ if (e.value != "") { if (!re.test(e.value)) { alert("请输入正确的数字"); e.value = ""; e.focus(); } } }*/ /* window.onload = function(){ $("#quality_unit").html($("#quality").val()); $("#concentration_unit").html($("#concentration").val()+"/"+$("#volume").val()); $("#volume_unit").html($("#volume").val()); $("#molecular_weight_unit").html($("#quality").val()+"/"+$("#concentration").val()); } function change(id,value,other=false){ if(other){ $("#"+id+"_unit").html(value+"/"+$("#"+other).val()); }else{ $("#"+id+"_unit").html(value); } }*/ function Calculation() { var flag = 0; var mass = ''; var concentration = ''; var volume = ''; var MolWt = ''; $(".cal_input").each(function () { if ($(this).val().replace(/\s+/g, '') != '') { flag++; } }); if ($("#MolWt").val() == "") { alert("请输入分子量的值！"); } else if (flag == 3) { var MassUnits = parseInt($("#MassUnits").val()); var ConcUnits = parseInt($("#ConcUnits").val()); var VolUnits = parseInt($("#VolUnits").val()); var MolWt = parseFloat($("#MolWt").val()); if ($("#mass").val() != '') { var mass = parseFloat($("#mass").val()) * Math.pow(10, MassUnits); } if ($("#concentration").val() != '') { var conc = parseFloat($("#concentration").val()) * Math.pow(10, ConcUnits); } if ($("#volume").val() != '') { var vol = parseFloat($("#volume").val()) * Math.pow(10, VolUnits); } if ($("#mass").val() == '') { var mass = conc * vol * MolWt; $("#mass").val(mass * Math.pow(10, -MassUnits)); } else if ($("#concentration").val() == '') { var moles = mass / MolWt; var conc = moles / vol; $("#concentration").val(conc * Math.pow(10, -ConcUnits)); } else if ($("#volume").val() == '') { var moles = mass / MolWt; var vol = moles / conc; $("#volume").val(vol * Math.pow(10, -VolUnits)) } } else { alert("没有足够的信息来执行计算！"); return false; } } pg ng μg mg g kg fM pM nM μM mM m nL μL mL L