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新型光控化学小分子交联剂-含NHS酯基和光保护ortho-QM(o-QM)的双功能交联剂NHQM
发布时间:2021-03-26     作者:wyf   分享到:

新型光控化学小分子交联剂-含NHS酯基和光保护ortho-QM(o-QM)的双功能交联剂NHQM

小分子交联剂对于探测生物分子间的相互作用和在处理大型蛋白复合物和内在无序蛋白时的交联质谱(CXMS)是非常宝贵的。现有的化学交联剂只针对一小部分氨基酸残基,限制了交联的数量和类型,而传统的光交联剂几乎不选择性地针对所有残基,使数据分析变得复杂。

本文设计合成了基于光保护的喹酮甲基化(PQM)的一系列交联剂,能够通过特定的Michael加成靶向9个亲核残基。除了AspGluLysSerThrTyr外,PQM交联剂还显著地交联了GlnArgAsn,迄今为止,这些化学交联剂还不能作为靶点,用单一交联剂显著地增加了靶点残基的数量。

 

较初设计了一种含NHS酯基和光保护ortho-QM(o-QM)的双功能交联剂NHQM。邻硝基苄基在酚羟基上的邻位甲基上连接了两个氟原子,当加入到蛋白中时,NHS会很快和暴露的Lys残基反应,将光保护的o-QM放置在相互作用蛋白的残基旁边,o-硝基苄基在紫外线照射下被清除,生成o-QM,并与附近的亲核侧链发生交联。

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为了增加交联剂的灵活性,还合成了NHQM3C,在NHQMspacer 中添加了三个亚甲基。实验结果表明,NHQMNHQM3C用小分子交联剂将自然残基的靶标库扩大到9个亲核残基。为了使细胞内高效的交联成为可能,还设计了一个具有同功能的交联剂HoQM,在两端都采用了光保护的o-QM。认为,将NHS酯替换为光保护的QM可以在光激活前消除交联剂的化学反应性,从而使其进入细胞而不会引起细胞毒性。此外,两端的QM可以增加交联类型的多样性,因为QMNHS酯相比会和更多的残基反应。

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DNA交联的试剂仍然很少,因为大多数交联剂的反应更倾向于蛋白-蛋白而不是蛋白-DNA交联。甲醛是主要的试剂交联试剂,但只能和在2埃以内的蛋白发生交联。QM能有效地烷基化脱氧核苷,因此,推断NHQM应该能够使蛋白质与DNA交联,实验证明的假设是正确的。

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  总之,这些PQM交联剂的光活化和多靶点反应性将大大增强蛋白质-蛋白质、蛋白质- DNA网络研究和结构生物学研究的化学交联基础技术。

齐岳生物供应:

产品列表:

DHB

2,5-dihydroxybenzoic acid

CAS Number: 490-79-9

CHCA

α-Cyano-4-hydroxycinnamic acid

CAS Number: 28166-41-8

HABA

2-(4'-Hydroxybenzeneazo)benzoic acid

CAS Number: 1634-82-8

Sinapinic Acid  

CAS Number: 530-59-6

THAP

2',4',6'-Trihydroxyacetophenone monohydrate

CAS Number: 480-66-0

DHAP

2',4',-Dihydroxyacetophenone

CAS Number: 89-84-9

MBT

MBT MALDI matrix, 2-Mercaptobenzothiazole

CAS Number: 149-30-4

 

 

色谱相关试剂

产品列表:

LCMS Grade Solvents

LCMS Acetonitrile     CAS Number: 75-05-8

LCMS Acetonitrile with 0.1% Acetic Acid

LCMS Acetonitrile with 0.1% Formic Acid

LCMS Acetonitrile with 0.1% TFA

LCMS Acetonitrile with 0.1% Formic Acid and 0.01% TFA

LCMS Water with 0.1% Acetic Acid

LCMS Water with 0.1% Formic Acid

LCMS Methanol       CAS Number: 67-56-1

LCMS Water          CAS Number: 7732-18-5

HPLC Grade Solvents

HPLC Acetone           CAS Number: 67-64-1

HPLC Acetonitrile        CAS Number: 75-05-8

HPLC Acetonitrile with 0.1% TFA

HPLC Cyclohexane       CAS Number: 110-82-7

HPLC Dimethyl sulfoxide  CAS Number: 67-68-5

HPLC Methanol          CAS Number: 67-56-1

HPLC Water             CAS Number: 7732-18-5

Acetic Acid Ampules (Proteomics Grade)    CAS Number: 64-19-7

Formic Acid Ampules (Proteomics Grade)    CAS Number: 64-18-6

TFA Ampules (Proteomics Grade)          CAS Number: 76-05-1

HFBA Ampules (Proteomics Grade)         CAS Number: 375-22-4

Formic Acid + TFA Ampules (Proteomics Grade)

LCMS Mobile Phases Sampler Pack

Additives & Ion Pairing

 

Proteochem蛋白交联试剂,进口蛋白交联试剂

Protein crosslinkers (蛋白交联相关试剂):

Heterobifunctional Crosslinkers异型双功能交联剂:

SMCC Crosslinker

Succinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate

CAS Number: 64987-85-5

Sulfo-SMCC Crosslinker

Sulfosuccinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate

CAS Number: 92921-24-9

Sulfo-SANPAH Crosslinker

N-Sulfosuccinimidyl-6-[4'-azido-2'-nitrophenylamino] hexanoate

CAS Number: 102568-43-4

SMPH Crosslinker

Succinimidyl-6-[ß-maleimidopropionamido]hexanoate

PDPH Crosslinker

3-[2-Pyridyldithio]propionyl hydrazide) is a cleavable, carbohydrate reactive

MBS Crosslinker

m-Maleimidobenzoyl-N-hydroxysuccinimide ester

CAS Number: 58626-38-3

Sulfo-MBS Crosslinker

m-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester

SPDP Crosslinker

N-Succinimidyl 3-[2-pyridyldithio]-propionate

CAS Number: 68181-17-9

LC-SPDP Crosslinker  

Succinimidyl 6-(3-[2-pyridyldithio]-propionamido)hexanoate

Sulfo-LC-SPDP Crosslinker

Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate

SIA Crosslinker

N-Succinimidyl iodoacetate

CAS Number: 39028-27-8

SBA Crosslinker

N-Succinimidyl bromoacetate; SIB crosslinker

CAS Number: 42014-51-7

SMPB Crosslinker

N-Succinimidyl 4-[4-maleimidophenyl]butyrate

CAS Number: 79886-55-8

BMPS Crosslinker

N-(ß-Maleimidopropyloxy)succinimide ester

CAS Number: 55750-62-4

EMCS Crosslinker

N-[e-Maleimidocaproyloxy]succinimide ester

CAS Number: 55750-63-5

GMBS Crosslinker

N-[Gamma-Maleimidobutyryloxy] Succinimide

CAS Number: 80307-12-6

ANB-NOS Crosslinker

N-5-Azido-2-nitrobenzoyloxysuccinimide

CAS Number: 60117-35-3

Homobifunctional Crosslinkers同型双功能交联剂:

BS3 Crosslinker

Bis[sulfosuccinimidyl] suberate

CAS Number: 82436-77-9

DSS Crosslinker

Disuccinimidyl suberate   N-hydroxysuccinimide ester

CAS Number: 68528-80-3

BS2G Crosslinker

BS2G; Bis[Sulfosuccinimidyl] glutarate

DSG Crosslinker

Disuccinimidyl glutarate; Di(N-succinimidyl

CAS Number: 79642-50-5

DSP Crosslinker

3,3'-Dithiodipropionic acid di(N-hydroxysuccinimide ester)

CAS Number: 57757-57-0

DTSSP Crosslinker

3,3´-Dithiobis[sulfosuccinimidylpropionate

CAS Number: 81069-02-5

Sulfo-EGS Crosslinker

glycolbis(sulfosuccinimidylsuccinate)

CAS Number: 167410-92-6

EGS Crosslinker

Ethylene glycolbis(succinimidylsuccinate)

CAS Number: 70539-42-3

DST Crosslinker  

CAS Number: 62069-75-4

Zero-Length Crosslinkers - EDC-HCl, Sulfo-NHS & NHS

EDC-HCl

1- Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride

CAS Number: 25952-53-8

Sulfo-NHS

N-hydroxysulfosuccinimide  Hydroxy-2,5-dioxopyrrolidine-3-sulfonicacid

CAS Number: 106627-54-7

上述产品仅用于科研,不可用于人体实验!

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